Pvuii takara
WebApr 19, 2013 · P53CON fragment long 474 bp was prepared by PvuII (TAKARA) digestion of supercoiled plasmid pPGM1 (pBSK containing a p53CON sequence AGACATGCCTAGACATGCCT) as described in [19], second fragment long 2513 bp was used as nonspecific competitor. WebThe 3.5-kb plasmid pHIG5-3 (2 .mu.g) was dissolved in a total volume of 50 .mu.l of a buffer containing 20 mM Tris-HCl (pH 7.5), 10 mM MgCl.sub.2, 10 mM dithiothreitol and 50 mM NaCl (hereinafter referred to as "Y-50 buffer"), and 4 units of the restriction enzyme PvuII (Takara Shuzo; hereinafter, unless otherwise specified, all the enzymes ...
Pvuii takara
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WebIt was confirmed that a recombinant plasmid derived from pHIG5-3 by insertion of the BamHI linker at the PvuII site had been ... 5 mM dithiothreitol, 0.1 mM EDTA and 1 mM ATP, 30 units of T4 polynucleotide kinase (Takara Shuzo) were added, and phosphorylation reaction was carried out at 37° C. for 60 minutes. Then, 0.4 μg of the pILAl ... WebJun 6, 2016 · MCRS1 is involved in multiple cellular activities, including mitotic spindle assembly, mTOR signaling and tumorigenesis. Although MCRS1 has been reported to bind to the dynein regulator NDE1, a ...
Web2 sigma-aldrich.com Table 4: Percentage Activity Enzyme AB L M H Aat II Acc I Acs I Acy I Afl III Alu I Alw 44 I Apa I Asp I Asp 700 Asp 718 Asp EI Ava I Ava II Avi II Bam HI Ban I Bbr PI Bcl I Bfr I Bgl I Bgl II Bln I Bmy I Bpu AI Bse AI Bsi WI Bsi YI Bsm I Bsp LU 11 Bss HII Bst 1107 Bst EII Bst XI Cel II WebBamHI at PvuII and hybridized with full length L1CAM cDNA. Southern blot analysis indicates no difference among the lanes Fig.3 Sequence from exon 8 amplified using …
Web欧阳超，刘思珍，满益龙，盛家伟，陈岳，张鑫，刘勇，张德咏，谭新球. 摘要：【目的】基于辣椒炭疽病菌種群复杂、田间复合侵染种群组成不清，导致防控相对困难的问题，建立一种快速直观的辣椒炭疽病菌区分标记系统，为其病害田间防控提供科学依据。 WebPlasmid phGH526 was then digested with restriction endonucleases PvuII (Takara Shozo) and BglIII (Takara Shozo) and an about 2.4 kb DNA fragment (fragment I) and an about 5.4 kb DNA (fragment II) were isolated form the reaction mixture by agarose gel electrophoresis.
WebThe PvuII-HindIII fragment was modified with T4 DNA polymerase at its termini and thereto was linked a phosphorylated XbaI linker with T4 DNA ligase. After digesting the ligate with restriction enzyme XbaI (Takara #1093A), the cleaved fragments were subjected to 6 % of acrylamide gel electrophoresis to prepare a XbaI-XbaI fragment of about 350 bp.
WebSearch Products by Name, Cat #, CAS #, or Keyword. ×. Login / Register basis cordis jantungWeb4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM367 · Revised 3/23 www.promega.com 6. Additional Protocols for Selected Restriction Enzymes Promega scientists have tested a subset of restriction enzymes for compatibility with rapid … tagram logoWebTaKaRa pvuii g46 a83 f94 encoding plasmids Pvuii G46 A83 F94 Encoding Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 … ta graWebNEBuffer Activity Chart for Restriction Enzymes. New England Biolabs provides a color-coded 10X NEBuffer with every restriction endonuclease to ensure 100% activity. basis cpd organiserWebSep 16, 2024 · To obtain the 5′-flanking region of AeNPR1a, genomic DNA was digested with EcoRV, DraI, StuI, and PvuII (TaKaRa, Dalian, China). Then, the digested DNA were purified and ligated separately with Genome Walker adaptors using the BD Universal Genome Walker™ kit (Clontech, Mountain View, CA, USA) according to the … ta grantsWebTakara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a … ta gradWebAug 25, 2024 · 2．2．2载体的线性化 16 第二军医人学硕十学位论文 p113．7载体图谱U3PPT cPPT PvuII￥296 Kpnl 5269 pBluescrJptK SaclI 5042 WPRE Spel 252 ICAG—enhancer 洲一Lnwnearl9－promoter CHv．FwdprImer EGFP—C．primer EGFP 取10ugp113．7载体，按照如下体系进行酶切处理 Rgal3698 EGFP—N－primer 1019 … basi scout lombardia