Pheochromocytoma pc12 cells
WebOct 10, 2024 · Cell Culture. The rat pheochromocytoma cell line PC12 was obtained from the RIKEN Cell Bank (Tsukuba, Ibaraki, Japan). PC12 cells were maintained at 37 °C in a humidified atmosphere containing 5% CO 2 in Dulbecco’s modified Eagle’s Medium (DMEM) supplemented with 10% fetal calf serum (FCS), 10% horse serum (HS), penicillin, and … WebMar 31, 1995 · Nerve growth factor (NGF) induces both differentiation and survival of neurons by binding to the Trk receptor protein tyrosine kinase. Although Ras is required for differentiation, it was not required for NGF-mediated survival of rat pheochromocytoma PC-12 cells in serum-free medium.
Pheochromocytoma pc12 cells
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WebOct 8, 2024 · Rat Pheochromocytoma PC12 Cells in Culture 1 Introduction. Two types of vesicles, synaptic vesicles (SVs) and dense-core vesicles (DCVs), have been found in … WebMar 19, 2024 · PC12 cell line was cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin, and incubated at 37°C in an atmosphere of 5% CO 2. The cells were then seeded in 96-well plates at a density of 5000 cells per well and detached using 0.25% Trypsin-EDTA after reaching 90% confluency for subculturing.
WebThe catecholamine-secreting PC12 cell line derived from a rat pheochromoytoma originating from chromaffin cells of the adrenal medulla has long been considered a suitable model system for studying neurosecretion and neuronal differentiation. The PC12 cell line has been used to get more information about diseases of the brain. It has been used in research of hypoxia, where acute hypoxia induces exocytosis and prolonged hypoxia can induce excessive exocytosis. PC12 cells were used to find which prion protein fragments caused neuronal dysfunction. In … See more PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla, that have an embryonic origin from the neural crest that has a mixture of neuroblastic cells and eosinophilic cells. See more This cell line was first cultured by Greene and Tischler in 1976. It was developed in parallel to the adrenal chromaffin cell model because of its extreme versatility for pharmacological manipulation, ease of culture, and the large amount of information on their … See more Research has shown differences in vesicle size and quantal size depending on treatment with certain drugs. • L-DOPA has shown increase in average quantal size when treated for only 40–90 minutes. • Treatment with amphetamine or reserpine causes … See more • Wikipedia articles containing the term "PC12 cells" [1] • Ras function was partially elucidated using PC12 cell line [2], from section in Cancer Medicine textbook See more Their embryological origin with neuroblastic cells means they can easily differentiate into neuron-like cells even though they are not considered adult neurons. Neuron-like means they share properties similar to neurons, in this case it is referring to releasing … See more PC12 cells have been researched to evaluate the potential for pharmaceutical alternation of dopamine metabolites such as DOPAL, an autotoxin implicated in the pathology of See more • Cell culture • Adrenal medulla • Cellular differentiation • Pheochromocytoma See more
WebMar 18, 2024 · This study aimed to determine the effects of reperfusion in an in vitro ischaemic stroke model—oxygen and glucose deprivation (OGD) (0.3% O2)—with rat pheochromocytoma (PC12) cells and cortical neurons. In PC12 cells, OGD resulted in a time-dependent increase in cytotoxicity and apoptosis, and reduction in MTT activity from … WebApr 14, 2024 · The PC12 pheochromocytoma cell line of neoplastic chromaffin cells was established from a rat adrenal tumour in 1976 1. Treatment of these cells with nerve …
WebJan 1, 1987 · This chapter presents several details regarding its culture and experimental exploitation—in particular, methods for the maintenance and nerve growth factor (NGF) …
WebPC12 cell line is well documented and widely applied as many kinds of models in neurobiological and neurochemical studies. Yet a thorough proteomic analysis has not … thin brick veneer whiteWebJun 14, 2013 · Tunneling nanotubes (TNTs) are thin cell-to-cell connected membranous tubes first described in rat pheochromocytoma (PC12) cells. 1 Subsequent studies … thin brick veneer on hearth of fireplaceWebJan 1, 1997 · We examined effects of three structurally related pyridinium compounds, 1-methyl-4-phenylpyridinium (MPP+), paraquat, and 1-methyl-4-(4'-nitrophenyl) pyridinium (analog 1), on the energy metabolism in pheochromocytoma PC12 cells. MPP+ inhibited the intracellular NADH oxidation by the mitochondrial r … saints birthday party invitationsWebFurthermore, culturing of rat pheochromocytoma (PC-12) cells is also described, as these cells represent a useful and widely used cell line for in vitro neurotoxicological studies. less. Related articles. Based on techniques. Mouse Enteric Neuronal Cell Culture Yonggang Zhang & Wenhui Hu, 2024, Springer Protocols. saints birthday cakesWebDec 28, 2024 · We have identified FA as a molecule that inhibits apoptosis induced by hydrogen peroxide (H 2 O 2) or actinomycin D (ActD) in rat pheochromocytoma, PC12 cell. We also found that FA reduces H 2 O 2 -induced reactive oxygen species (ROS) production in PC12 cell, thereby acting as an anti-oxidant. Then, we analyzed FA-mediated signaling … saints birthday picWebThe PC12 clone, developed from a pheochromocytoma tumor of the rat adrenal medulla, has become a premiere model for the study of neuronal differentiation. saints birthday partyWebMix the culture medium containing detached PC12 cells with fresh complete medium in a 1:3 or 1:4 ratio. Plate the PC12 cells onto collagen-coated dishes, and increase the passage number of the newly plated PC12 cells by one. As the cell doubling time is 3-4 days, subculture every 7-10 days. saints birthdays in december